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Horizontal gene transfer is an important driver of bacterial evolution, but genetic exchange in the core genome of clonal species, including the major pathogen Staphylococcus aureus, is incompletely understood. Here we reveal widespread homologous recombination in S. aureus at the species level, in contrast to its near-complete absence between closely related strains. We discover a patchwork of hotspots and coldspots at fine scales falling against a backdrop of broad-scale trends in rate variation. Over megabases, homoplasy rates fluctuate 1.9-fold, peaking towards the origin-of-replication. Over kilobases, we find core recombination hotspots of up to 2.5-fold enrichment situated near fault lines in the genome associated with mobile elements. The strongest hotspots include regions flanking conjugative transposon ICE6013, the staphylococcal cassette chromosome (SCC) and genomic island νSaα. Mobile element-driven core genome transfer represents an opportunity for adaptation and challenges our understanding of the recombination landscape in predominantly clonal pathogens, with important implications for genotype-phenotype mapping.

Original publication

DOI

10.1038/ncomms4956

Type

Journal article

Journal

Nature communications

Publication Date

23/05/2014

Volume

5

Addresses

1] Nuffield Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford OX3 9DU, UK [2].

Keywords

Chromosomes, Bacterial, Staphylococcus aureus, DNA Transposable Elements, Likelihood Functions, Phylogeny, Species Specificity, Recombination, Genetic, Gene Transfer, Horizontal, Linkage Disequilibrium, Genome, Bacterial, Genetic Variation