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High-throughput technologies can identify genes whose expression profiles correlate with specific phenotypes; however, placing these genes into a biological context remains challenging. To help address this issue, we developed nested Expression Analysis Systematic Explorer (nEASE). nEASE complements traditional gene ontology enrichment approaches by determining statistically enriched gene ontology subterms within a list of genes based on co-annotation. Here, we overview an open-source software version of the nEASE algorithm. nEASE can be used either stand-alone or as part of a pathway discovery pipeline.nEASE is implemented within the Multiple Experiment Viewer software package available at data are available at Bioinformatics online.

Original publication




Journal article


Bioinformatics (Oxford, England)

Publication Date





726 - 728


Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Harvard School of Public Health, Boston, MA, USA.


Humans, Neoplasms, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Algorithms, Software, Vocabulary, Controlled