Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The development of aptamers on custom synthesized DNA microarrays, which has been demonstrated in recent publications, can facilitate detailed analyses of sequence and fitness relationships. Here we use the technique to observe the paths taken through sequence-fitness space by three different evolutionary regimes: asexual reproduction, recombination and model-based evolution. The different evolutionary runs are made on the same array chip in triplicate, each one starting from a small population initialized independently at random. When evolving to a common target protein, glucose-6-phosphate dehydrogenase (G6PD), these nine distinct evolutionary runs are observed to develop aptamers with high affinity and to converge on the same motif not present in any of the starting populations. Regime specific differences in the evolutions, such as speed of convergence, could also be observed.

Original publication

DOI

10.1088/1478-3975/7/3/036007

Type

Journal article

Journal

Physical biology

Publication Date

09/2010

Volume

7

Addresses

Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester, UK. william.rowe@manchester.ac.uk

Keywords

Glucosephosphate Dehydrogenase, Oligonucleotide Array Sequence Analysis, Algorithms, Models, Genetic, Computer Simulation, Aptamers, Nucleotide