Alternative splicing plays a major role in regulating the functional repertoire of the proteome. However, isoform-specific effects to protein-protein interactions (PPIs) are usually overlooked, making it impossible to judge the functional role of individual exons on a systems biology level. We overcome this barrier by integrating protein-protein interactions, domain-domain interactions and residue-level interactions information to lift exon expression analysis to a network level. Our user-friendly database DIGGER is available at https://exbio.wzw.tum.de/digger and allows users to seamlessly switch between isoform and exon-centric views of the interactome and to extract sub-networks of relevant isoforms, making it an essential resource for studying mechanistic consequences of alternative splicing.
Journal article
2021-01-01T00:00:00+00:00
49
D309 - D318
Chair of Experimental Bioinformatics, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany.
Humans, Protein Isoforms, Proteome, RNA, Messenger, Protein Interaction Mapping, Computational Biology, Protein Biosynthesis, Alternative Splicing, Binding Sites, Protein Binding, Exons, Thermodynamics, Models, Molecular, Internet, Software, Databases, Protein, Protein Interaction Domains and Motifs, Protein Conformation, alpha-Helical, Protein Conformation, beta-Strand