We describe a rapid method of HLA class I typing using the polymerase chain reaction and oligonucleotide hybridisation that eliminates the requirements for viable lymphocytes and allows subtypes to be defined. We have used this to demonstrate that the predominant subtype of HLA-B27 in the Gambia, West Africa, is HLA-B*2703, which is very rare or absent in other racial groups. This subtype differs from the common Caucasian HLA-B27 subtypes in its recognition by cytotoxic T cells. We propose that HLA*B-2703, unlike other HLA-B27 subtypes, may not be associated with ankylosing spondylitis, thus accounting in part for the rarity of this condition in black populations.

Original publication

DOI

10.1016/0140-6736(91)92452-8

Type

Journal article

Journal

Lancet (London, England)

Publication Date

03/1991

Volume

337

Pages

640 - 642

Addresses

Molecular Immunology Group, University of Oxford, John Radcliffe Hospital.

Keywords

Humans, HLA-B27 Antigen, Histocompatibility Testing, Polymerase Chain Reaction, Nucleic Acid Hybridization, Base Sequence, Alleles, Molecular Sequence Data, Adult, Child, African Continental Ancestry Group, Gambia, Evaluation Studies as Topic