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B cells undergo rapid mutation and selection for antibody binding affinity when producing antibodies capable of neutralizing pathogens. This evolutionary process can be intermixed with migration between tissues, differentiation between cellular subsets, and switching between functional isotypes. B cell receptor (BCR) sequence data has the potential to elucidate important information about these processes. However, there is currently no robust, generalizable framework for making such inferences from BCR sequence data. To address this, we develop three parsimony-based summary statistics to characterize migration, differentiation, and isotype switching along B cell phylogenetic trees. We use simulations to demonstrate the effectiveness of this approach. We then use this framework to infer patterns of cellular differentiation and isotype switching from high throughput BCR sequence datasets obtained from patients in a study of HIV infection and a study of food allergy. These methods are implemented in the R package dowser, available at https://dowser.readthedocs.io.

Original publication

DOI

10.1371/journal.pcbi.1009885

Type

Journal article

Journal

PLoS computational biology

Publication Date

25/04/2022

Volume

18

Addresses

Department of Pathology, Yale School of Medicine, New Haven, Connecticut, United States of America.

Keywords

B-Lymphocytes, Humans, HIV Infections, Receptors, Antigen, B-Cell, Phylogeny, Cell Differentiation, Immunoglobulin Class Switching