In humans, most meiotic crossover events are clustered into short regions of the genome known as recombination hot spots. We have previously identified DNA motifs that are enriched in hot spots, particularly the 7-mer CCTCCCT. Here we use the increased hot-spot resolution afforded by the Phase 2 HapMap and novel search methods to identify an extended family of motifs based around the degenerate 13-mer CCNCCNTNNCCNC, which is critical in recruiting crossover events to at least 40% of all human hot spots and which operates on diverse genetic backgrounds in both sexes. Furthermore, these motifs are found in hypervariable minisatellites and are clustered in the breakpoint regions of both disease-causing nonallelic homologous recombination hot spots and common mitochondrial deletion hot spots, implicating the motif as a driver of genome instability.

Original publication

DOI

10.1038/ng.213

Type

Journal article

Journal

Nature genetics

Publication Date

09/2008

Volume

40

Pages

1124 - 1129

Addresses

Broad Institute of Massachusetts Institute of Technology and Harvard, 7 Cambridge Center, Cambridge, Massachusetts 02142, USA. myers@stats.ox.ac.uk

Keywords

Humans, Genomic Instability, Recombination, Genetic, Crossing Over, Genetic, Base Sequence, Repetitive Sequences, Nucleic Acid, Mutation, Molecular Sequence Data